Fig. 3

C. albicans cerebritis induces upregulation of amyloid precursor protein (APP). Mice were challenged with C. albicans as in Fig. 2 and RNA and protein extracted from whole brain. a qRT-PCR was performed to quantify mRNA for APP over 14 days. b Western blot analysis of APP over the same time period. c Densitometric analysis of the western blot data. d Immunofluorescence images of brain from C. albicans-infected wild-type mice 4 days post i.v. challenge with C. albicans showing staining for DAPI, IBA1, APP, NeuN, and merged images. Images are centered on fungal granulomas similar to those shown in Fig. 2. e Repeat staining of fungal granulomas for DAPI, IBA1, APP, and calcofluor white (CW) comparing wild-type to app^−/− mice. (n = 4, mean ± S.E.M, *p < 0.05, **p < 0.01, and ***p < 0.001 using one-way ANOVA followed by Dunnett’s test for multiple comparison. Scale bar: 20 μm). Data are shown as representative of two independent experiments