Fig. 4 | Nature Communications

Fig. 4

From: Genome-wide association analyses of invasive pneumococcal isolates identify a missense bacterial mutation associated with meningitis

Fig. 4

Effects of pbp1bA641C on antibiotic resistance and tolerance in isogenic strains. a The pbp1b locus in the R6 strain was replaced by an allele carrying the pbp1bA641C point mutation to construct the R6_641C strain. b MIC test carried out using the penicillin E-test strip. c Survival of the isogeneic R6 and R6_641C strains in the presence of 3 μg ml-1penicillin (> 100-fold MIC). Data from eight independent experiments (Exp_1 to Exp_8) are shown. d Minimum duration for killing 99% of bacterial cells in the population (MDK99) estimated from the survival curves. Data are shown as mean ± SD from the eight experiments in c. Dot plots are overlaid in the bar charts to show MDK99 estimation from individual experiments. The P-value is based on a two-sample t test (two tailed)

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