Table 1 Properties of currently available monomeric NIR FPs designed from various bacterial photoreceptors

NIR FP	Parental bacterial photoreceptor	Ex (nm)	Em (nm)	Extinction coefficient (M⁻¹cm⁻¹)	Quantum yield (%)	Molecular brightness vs. miRFP670 (%)	Oligomeric state	pKa	Photostability in HeLa cells, t_1/2 (s)	Brightness in mammalian cells vs miRFP670 (%)^a	Ref.
miRFP670 nano	NpR3784 cyanobacteriochrome	645	670	95,000	10.8	84	Monomer	3.7	505	100^b	This work
miRFP670	RpBphP1 bacterial phytochrome	642	670	87,400	14.0	100	Monomer	4.5	183	100	³
miRFP703		674	703	90,900	8.6	64	Monomer	4.5	394	61
miRFP709		683	709	78,400	5.4	35	Monomer	4.5	192	42
miRFP720	RpBphP2 bacterial phytochrome	702	720	98,000	6.1	49	Monomer	4.5	510	160	⁵
mIFP	BrBphP bacterial phytochrome	683	704	82,000	8.4	56	Monomer	4.5	54	26	^{4, 8}
smURFP	TeAPCα allophycocyanin	642	670	180,000^c	18.0	265^c	Dimer	–	570	1	^{6, 8}
BDFP1.5	ApcF allophycocyanin	688	711	74,000	5.0	30	Monomer	2.0	1310^d	0.5^d	⁷

^aUnless otherwise stated, it is determined as effective NIR fluorescence in live HeLa cells 72 h after transfection with no supply of exogenous BV and after normalization to fluorescence of co-transfected EGFP
^bEffective brightness of miRFP670nano in various mammalian cell lines is compared in Fig. 2e
^cDetermined for a dimer of smURFP molecules
^dBased on the comparison with smURFP in HEK293 cells in ref. ⁷

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