Fig. 2

Bone marrow-derived monocytes contribute to the mammary gland macrophage pool after birth. a Flow cytometric analyses of mammary gland (MG) macrophage populations in wild type (WT) and Ccr2^−/− mice. Shown are representative FACS plots and quantifications of F4/80^Int and F4/80^Hi macrophage populations at the indicated time points. b The experimental outline for depleting macrophages with anti-CSF1 and clodronate treatment. i.p., intraperitoneal; i.v., intravenous. IgG, isotype-matched control antibody. c Analysis of total F4/80^Int and F4/80^Hi macrophage numbers in the MG of clodronate-anti-CSF1 treated (Clod + aCSF1) and control treated (CO) mice one day after the last treatment (at day 24). d Analyses of frequencies of F4/80^Int and F4/80^Hi macrophage populations in MG of Clod + aCSF1 and CO treated mice after an 11 day recovery period (at 5 wk). In a, c, and d MG macrophages were pre-gated as live CD45⁺CD11b⁺Siglec-F⁻ cells. In a, c and d F4/80^Int macrophages have been gated in blue (newborn mice) or in black (2 wk – 3 months old mice), and the F4/80^Hi macrophages in red (newborn mice) or orange (2 wk – 3 months old mice). In the quantifications, each dot represents one mouse and mean ± SEM are shown. Data are from 1 (a newborn), 2 (a 2 wk, c, d) and 3 (a 5 wk and 3 months) independent experiments. *p < 0.05, **p < 0.01, ***p < 0.001 (Kruskal–Wallis test). Source data are provided as a Source Data file