Fig. 5

CD206^Hi macrophages show distinct functions and location in adult mammary glands. a Whole mount carmine alumine stainings of mammary gland (MG) and quantification (branch number and ductal area) of the branching morphogenesis in 5 wk old mice of the indicated genotypes. LN, lymph node. b Uptake of intravenously administered fluorescent 500 kDa dextran, acetylated LDL and ovalbumin-anti-ovalbumin antibody immunocomplexes by CD206^Neg/low and CD206^Hi macrophage subpopulations in the MG of 5 wk old wild type (WT) mice. Control mice received PBS injections. MFI, specific mean fluorescence intensity. c Immunohistochemical analysis of CD206, F4/80, and Siglec-F expression in the MG of 5 wk old WT mice. Representative CD206⁺F4/80⁺Siglec-F⁻ macrophages (light blue arrows), CD206⁻F4/80⁺Siglec-F⁻ macrophages (magenta arrows) and CD206⁻F4/80⁺Siglec-F⁺ eosinophils (orange arrows) are pointed out. Blue is Hoechst. Bar, 50 µm. d Analysis of CD206, F4/80 and alpha smooth muscle actin (αSMA) expression in the MG. An orange arrow points to a representative duct. Blue is DAPI. Bar, 25 µm. e Analysis of CD206, F4/80, and CD31 expression in the MG. A light blue arrow points to a representative vessel, and an orange arrow to a representative duct. Blue is DAPI. Bar, 25 µm. f Microscopic analyses of CD206 and CD31 in MG of mice injected intravenously with fluorescent dextran (yellow). Transmural projections of CD206⁺ macrophages, which have ingested dextran, are pointed out by a light blue arrow. Bar, 10 µm. In b MG macrophages were defined as CD45⁺CD11b⁺Siglec-F⁻ cells. In the quantifications, each dot represents one mouse and mean ± SEM are shown. Quantitative data are from three (a, b OVA-SIC) and two (b Dextran and LDL) independent experiments. c–f are representative figures from three independent experiments *p < 0.05, **p < 0.01, ***p < 0.001 (Kruskal–Wallis test). Source data are provided as a Source Data file