Fig. 3

Immunoglobulins bound to circulating plasma exosomes. a Schematic of the work-flow used for the isolation and Ig quantification of plasma exosomes from plasma of PDAC patients and healthy controls. b Flow-cytometry analysis using anti-human IgG of exosomes isolated from the plasma of cancer patients coupled to 0.4 μm-diameter beads. The graphs show the representative analysis of two PDAC patient samples. c Table showing the relative spectral abundancy (ion intensity) in PDAC and healthy subject plasma exosomes of immunoglobulins. Numbers were normalized based on the number of exosomes (30–200 nm size) in each sample, as quantified by nanoparticle-tracking. Inf indicates proteins uniquely identified in cancer patient exosomes