Fig. 1

LEF-10-EGFP forms aggregates in infected insect cells. a Diagram of the recombinant baculovirus sensor system functions by transmitting the aggregation state of LEF-10-EGFP to downstream gene reporter mCherry outputs. The cassette fragment was integrated into the polh locus of a lef-10 knock-out Bacmid via site-specific homologous recombination (Supplementary Fig. 1a). b LEF-10-EGFP exists in two distinct forms: as aggregated (Cell 1) or as diffuse (Cell 2) forms in infected insect cells (MOI = 3) at 60 hpi (left). The mCherry reporter protein representing late gene expression can only be detected in cells harboring diffuse LEF-10-EGFP (right). c The distribution of LEF-10-EGFP in the two cells (b, left) was analyzed by ImageJ software. The proportion of the pixels of a certain brightness to all the pixels harbored by one cell is defined as “percentage of pixel”. Higher fluorescence intensity, which leads to the curve shifting to the right in Cell 1, indicates the aggregation of LEF-10-EGFP and exhausts the pool of non-aggregated LEF-10-EGFP which occurs in the lower fluorescence intensity areas