Fig. 2 | Nature Communications

Fig. 2

From: Loss-of-function mutations in QRICH2 cause male infertility with multiple morphological abnormalities of the sperm flagella

Fig. 2

QRICH2 is detected in human sperm flagella. a–i 3D STORM images of QRICH2 (green) (a–c), α-Tubulin (red) (d–f) and their co-localization (yellow) (g–i). a, d, g, x-y-z projections (scale bars, 5 µm). b, e, h, x–y projections: sections surrounded by white square (scale bars, 0.5 µm). c, f, i, y-z cross-sections near the annulus (white arrowheads) (scale bars, 0.1 µm). j The domains of QRICH2 and the positions of two mutations in QRICH2. QRICH2 contains three domains: Glutenin hmw super family (amino acid positions 282–864), SMC_N super family (amino acid positions 1036–1402) and DUF4795 (amino acid positions 1252–1432). k Immunofluorescence staining of QRICH2 on human spermatozoa from normal control and the two MMAF individuals. QRICH2 staining (red) was absent in sperm from PA and significantly weak in sperm from PB. (blue, DAPI; scale bars, 5 µm)

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