Fig. 3

Correlative STORM/EM analysis positions DAP signals with respect to EM densities. a RPE-1 C1-GFP cells were immunolabeled for the indicated DAPs and imaged first in a wide-field mode (magenta in inserts), followed by 3D STORM imaging, and correlative electron microscopy analysis. The panels show one 80 nm section containing DA EM densities, the STORM image of the same centriole, merged STORM and EM image, and the STORM image with a scheme delineating the centriole and the DA head densities. The averaged STORM signal is shown in the right column. Averaged signals were pseudo colored. b Averaged STORM images from (a) were rotated and then superimposed to generate a horizontal distributional map of the DAPs. c, d Two protein STORM analysis of indicated DAPs. Cells were simultaneously labeled for two DAPs in one channel and imaged by STORM. The averaged signals from all nine appendages are shown below. More examples of two-protein STORM are shown in Supplementary Figure 4, where the average outer and inner diameter of the toroid is noted. Scale bars: 400 nm