Fig. 6
From: Structural basis for species-selective targeting of Hsp90 in a pathogenic fungus
Species-selective antifungal activity of Hsp90 inhibitor CMLD013075. a Relative growth inhibition by Hsp90 inhibitors of reference C. albicans strain (SN95) and a clinical isolate (CaCi-2) with resistance to FL. The effect of 48-hour exposure to each inhibitor over a twofold dilution series of concentrations is displayed in heat-map format. Each colored box represents the mean of duplicate determinations. Color scale for relative growth is provided to far right of panel, green: no inhibition to black: complete inhibition. The experiment was repeated as an independent biological replicate to confirm results. b Concentration-dependent inhibition of mammalian cell growth and survival after 3-day culture with Hsp90 inhibitors. Data points depict the mean of triplicate determinations, error bars, SD. The experiment was repeated as an independent biological replicate to confirm results. c Monolayer cultures of human HEPG2 cells in 6-well format were infected with C. albicans and then incubated with the indicated compounds for 48 h (FL, conventional antifungal fluconazole). Cultures were then fixed and stained by Periodic Acid-Schiff (PAS) technique to visualize fungal burden (dark red/purple signal) and underlying human cells (light pink signal). Representative fields from conventional light photomicrographs are presented at the same magnification in all panels. The experiment was repeated in two independent biological replicates to confirm results. Scale bar, 100 μm
