Fig. 3
From: Efficient oral vaccination by bioengineering virus-like particles with protozoan surface proteins
VLP characterization. a Immunofluorescence microscopy of HEK cells used in VLPs production. The different DNA constructs used are indicated on top of each figure. In all immunofluorescence images, nuclei are labeled with DAPI (blue). The white signal represents colocalization. The bars represent 20 μm. b Electron micrographs of 100 nm-thick cryosections showing VLPs budding from the surface of HEK-1267 cells. Five nanometer immunogold labeling of HA (top panels) and VSP1267 (bottom panels) indicates their localization in cells and VLPs membranes (arrows). N, nucleus. The bars represent 100 nm. c Western blotting and hemagglutination assays showing the correct assembly of VLPs. d Representative TEM negative staining of VLPs (VLP-HA on the left and VLP-HA/VSP1267-G on the right). The bars represent 100 nm. e Immunofluorescence images of VSPs on the surface of Giardia trophozoites (left) and of VSP-G on the surface of VLPs (right). In both cases, mAb 7F5-FITC anti-VSP1267 extracellular domain was used. VSPs are labeled in green and nuclei stained with DAPI in blue. The bars represent 25 μm. Source data are provided as a Source Data file
