Fig. 2
From: Gigaxonin E3 ligase governs ATG16L1 turnover to control autophagosome production
Gigaxonin induces the degradation of ATG16L1, through the proteasome and the lysosomal pathways. a Immunofluorescence of COS-7 cells transfected with Flag-Gigaxonin and Cherry-ATG16L1 alone (single transfection) or together (double transfection), in basal condition, or treated with MG132 or Bafilomycin A1 (Baf). Gigaxonin-dependent degradation of ATG16L1 is rescued upon both treatments. b Immunoblotting of cell extracts prepared as in a to show the proteasome and autophagy-dependent degradation of ATG16L1 by Gigaxonin. Immunofluorescence (c) and immunoblotting (d) showed that endogenous ATG16L1 protein is greatly diminished in Gigaxonin transfected cells, and partially restored under MG132 or Bafilomycin A1 treatment. Scale bar: 10 µm and 50 µm for zoom pictures
