Fig. 6
From: Gigaxonin E3 ligase governs ATG16L1 turnover to control autophagosome production
Early and late autophagy steps are rescued by lentiviral-Gigaxonin in GAN-/- neurons. Immunostaining (a) and immunoblots (c) for ATG16L1 were performed on 4 div control and mutant neurons, after transduction with mock-GFP or Flag-Gigaxonin lentivirus. Both normal and bundled ATG16L1 were cleared upon expression of Gigaxonin in control and GAN-/- neurons, respectively. b Gigaxonin expression restored p62 levels in mutant cells, supporting an effective maturation of autophagosomes and autophagy degradation. Scale bar: 20 µm
