Fig. 7

Herpes virus-associated ubiquitin-specific protease (HAUSP) and Yap are upregulated in hepatocellular carcinoma (HCC) samples. a, b The relative expression of HAUSP (a) and Yap (b) in 60 pairs of HCC and normal liver samples. Each bar was the log₂ value of the ratio of HAUSP or Yap protein levels between HCC (C) and matched normal tissues (N) from the same patient. The bar > 0 represented HAUSP was upregulated in HCC sample because log₂1=0, vice versa. c Comparison between HAUSP elevation and Yap elevation in HCC samples. The overlapping region represented both HAUSP and Yap elevation cases. d Relative mRNA levels of indicated genes from HCC samples and matched normal tissues were revealed by real-time PCR. e Relative mRNA levels of indicated genes from Huh7 cells were revealed by real-time PCR. f Relative mRNA levels of indicated genes from Huh7 cells were revealed by real-time PCR. g MTT results of Huh7 cells transfected with indicated constructs or siRNAs. h Immunoblots of lysates from Huh7 cells treated with Usp7 inhibitor P5091 at indicated concentrations for 24 h. Actin acts as a loading control. i Relative mRNA levels of indicated genes from Huh7 cells treated with P5091 at distinct concentrations for 24 h. j Cell proliferation of Huh7 cells treated with P5091 at indicated concentrations for 24 h. k Immunoblots of lysates from Huh7 cells treated with Usp7-IN-1 or HBX-19818 at indicated concentrations for 24 h. Actin acts as a loading control. l Usp7 inhibitors suppressed Yap ubiquitination. Quantative data are shown as means ± SEM. n = 3 biological-independent experiments. In all above results, ^**P < 0.01, ^***P < 0.001, ns, not significant by Student’s t-test