Fig. 1

Identification of differentially expressed host and parasite transcripts. a Hierarchical clustering of RNAseq samples based on gene-expression patterns in the indicated host hepatocytes and specified times postinfection. The color scale for the figure, as indicated by the scale bar in the lower left corner of Fig. 1a, indicates log₂ fold changes from −3 to +3. Data used to generate heatmap is located within Supplementary Data 3. b Volcano plot of gene-expression pattern vs. p value, with the position of MUC13 indicated. c Confirmation of host factor upregulation via qPCR, with relative quantitation of the indicated host transcripts calculated using the ΔΔCT method, in the indicated hepatocyte cell types (data presented as mean ± s.e.m, n = 3 with individual biological replicates overlaid, * = p value < 0.05, *** = p value < 0.001. p values determined by two-tailed t test). d Schematic of MUC13 protein structure and approximate antibody locations. Antibody #1: AbCam #Ab65109, Antibody #2: LifeSpan BioSciences #LS-C345092 (discontinued), and Antibody #3: LifeSpan BioSciences #LS-A8191. e Confirmation of MUC13 upregulation during infection via western blot