Fig. 2 | Nature Communications

Fig. 2

From: Dual RNA-seq identifies human mucosal immunity protein Mucin-13 as a hallmark of Plasmodium exoerythrocytic infection

Fig. 2

Expression and localization of MUC13 in Plasmodium infected hepatoma cells. a, b Confocal microscopy images of representative HC04 liver cells infected with Plasmodium parasites 48 hpi at two different magnifications. c Control Confocal microscopy images of representative HC04 liver cells infected with Plasmodium parasites 48 hpi without MUC13 antibody being used. Cells were labeled using a rabbit polyclonal antibody (dilution 1:500, 1 mg/ml stock) against the intracellular region of MUC13 (MUC13 antibody #1—AbCAM #Ab65109) and visualized with a goat anti-rabbit Alexa Fluor 488 (green); CellMask deep red was used for plasma membranes (Cell Membrane—magenta). P. berghei EEFs were labeled using a goat polyclonal (dilution 1:200, 1 mg/ml stock) against PbUIS4 (LS-204260, LifeSpan Biosciences) and visualized with a bovine anti-goat secondary antibody (Alexa Fluor 647, red), respectively. Nuclei were labeled with Hoechst 33342 (blue). Merged images between HsMUC13, UIS4 and Hoechst are shown. Scale bars 50 (zoom 1) or 10 (zoom 2) μm; 63× oil objective

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