Fig. 3
Temporal detection of MUC13 in HC04 cells infected with P. berghei. a HC04 cells were infected with P. berghei sporozoites and then fixed and stained at 2, 12, 24, 36, and 48 hpi. Infected cell cultures were stained using a 1:500 dilution (1 mg/ml stock) of mouse polyclonal antibody to P.spp HSP70 (see methods) and a 1:500 dilution of rabbit polyclonal antibody to MUC13 intracellular domain (MUC13 antibody #2—LifeSpan BioSciences #C345092). Primary antibody localization was visualized with goat anti-mouse (Alexa Fluor 647, red) and goat anti-rabbit (Alexa Fluor 488, green) secondary antibodies, respectively. Nuclei were stained with Hoechst 33342 (blue) and cell membranes with CellMask deep red (magenta). Scale bars 10 μm; 60× oil objective. b The total reads, from the Huh7.5.1 RNA seq samples 1–3, for MUC13 at the 5 indicated time points (0 h uninfected, 24 h infected, 24 h uninfected, 48 h infected, and 48 h uninfected). c The fold-induction of MUC13, based upon the total read count in panel B, at 24 and 48 hpi, presented as a ratio of Infected:Uninfected. Data presented as mean ± s.e.m, n = 3 with individual biological replicates overlaid
