Fig. 1

Cbfβ is not necessary for ILC2 differentiation. a Flow cytometry analysis of Runx1-GFP expression from the P1 promotor and Runx1-RFP from the P2 promotor by ILC2s (CD45⁺ CD3^– CD19^– CD127⁺ CD25⁺ ST2⁺) in the lungs of WT (top) and Runx1^{+/P1-GFP: P2-RFP} mice (bottom). b Gross appearance of the Cbfb^+/f PLZF-Cre (left) and Cbfb^f/f PLZF-Cre mice (right). c Quantitative PCR analysis of the relative copy numbers of the floxed genes (loxP site, loxps) located in Cbfb intron 4 compared to those of the unfloxed genes in Cbfb intron 4 in the indicated cell populations of Cbfb^f/f PLZF-Cre mice. d RT-PCR assay of Cbfb transcripts with or without a mutation in the indicated cell populations of the Cbfb^+/f PLZF-Cre (1) and Cbfb^f/f PLZF-Cre mice (2). e Quantitative PCR analysis of the relative copy numbers of Cbfb transcripts from the floxed Cbfb gene locus in the indicated cell populations of Cbfb^f/f PLZF-Cre mice. f, g Chimerism of common lymphoid progenitor (CLP: CD45⁺ Lin^– cKit^lo CD127⁺ sca1^lo Flt3⁺ α4β7^–), CHILP (CD45⁺ Lin^– CD127⁺ α4β7⁺ Flt3^– CD25^–), PLZF⁺ ILCP (CD45⁺ Lin^– cKit⁺ CD127⁺ α4β7⁺ PLZF-GFP⁺), ILC2 precursor (ILC2P: CD45⁺ Lin^– CD127⁺ α4β7⁺ Flt3^– CD25⁺) cells, ILC1s (CD45⁺ CD3^– CD19^– NK1.1⁺ NKp46⁺ CD127⁺), ILC2s (CD45⁺ CD3^– CD19^– CD127⁺ GATA-3⁺), and NCR^{+ or –} (NKp46^{+or –}) ILC3s (CD45⁺ CD3^– CD19^– CD127⁺ RORγt⁺), NK cells, CD4⁺ T cells, CD8⁺ T cells, CD19⁺ B cells, neutrophils, eosinophils, basophils, and DCs (CD11b⁺ CD11c⁺) from the indicated tissues of lethally irradiated host mice (CD45.1⁺) reconstituted with a mixture (1:1) of bone marrow cells from the wild type (CD45.1⁺) and Cbfb^+/f PLZF-Cre or Cbfb^f/f PLZF-Cre (CD45.2⁺) mice. BM bone marrow, SI small intestine lamina propria lymphocytes. Numbers adjacent to the outlined areas in c indicate the percentages of CD45.1^–/CD45.2⁺ (mutant donor) or CD45.1⁺ CD45.2^– (wild type) cells. In g, **p < 0.01 and ***p < 0.001 by Student’s t-test. Data are representative of at least two independent experiments (mean ± s.d. of three technical replicates in c, e, mean ± s.d. of 4 mice in f, g)