Fig. 3
Runx proteins inhibit the gene expression regulated by GATA-3 in ILC2s at steady state. a Heat map of the expression of selected genes positively (left) or negatively (right) regulated by GATA-3 in ILC2s as determined by RNA sequence analysis. b Gene set enrichment analysis of genes positively (top) or negatively regulated by GATA-3 performed on a gene set that was differentially expressed in Cbfbf/f PLZF-Cre ILC2s compared to Cbfb+/f PLZF-Cre ILC2s. c, d Host mice (CD45.1+) were lethally irradiated and reconstituted with CD45.2+ bone marrow cells that were retrovirally transduced with the control vector, pMSCV-Thy1.1 (left) or pMSCV-Runx3-Thy1.1 (right). Flow cytometry analysis of IL-5 and IL-13 expression by untransduced Thy1.1– (top) or transduced Thy1.1+ (bottom) donor CD45.2+ ILC2s (CD45+ CD3– CD19– CD127+ CD25+ KLRG1+) from the lungs of the host mice (c). The frequency of IL-5-producing cells in the indicated ILC2s was determined by flow cytometry as in c (d). Numbers in quadrants of the bottom plots indicate the percentages of cells in each quadrant. In d, ***p < 0.001 by Student’s t-test. Data are representative of at least two independent experiments (mean ± s.d. of three mice in d)
