Fig. 6 | Nature Communications

Fig. 6

From: Runx/Cbfβ complexes protect group 2 innate lymphoid cells from exhausted-like hyporesponsiveness during allergic airway inflammation

Fig. 6

Enhancer and repressor activities of Runx proteins in ILC2s stimulated with IL-33. a Tracks of Cbfβ ChIP sequence traces in ILC2s cultured with IL-2 and IL-7 (top) and Cbfβ, GATA-3, H3K27 acetylation (ac), and H3K27 trimethylation (me3) ChIP sequence traces in ILC2s cultures with IL-2, IL-7, and IL-33 (bottom three). Asterisks(*) indicates a differential peak in IL2s cultured with IL-2, IL-7, and IL-33 compared to ILC2s cultured with IL-2 and IL-7. b Venn diagram showing the number of genes with Cbfβ binding peaks marked by H3K27ac peaks (left) and the downregulated genes in the Cbfβ-deficient ILC2s as determined by RNA sequence analysis (right). c Gene ontology enrichment analysis using the top 200 downregulated genes out of the 1009 genes in a (middle). Bar plots indicates the –log p value for GO term enrichment. d H3K27me3 binding to the Il10 promoter locus in the indicated mouse ILC2s cultured with IL-2. IL-7, and IL-33. In d, *p < 0.05 by Student’s t-test. Data are representative of two independent experiments (mean ± s.d. of three mice in d)

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