Fig. 7

Exhausted-like ILC2s appear at the severe inflammation site. a–f IL-10-Venus mice (a, b) or C57BL/6 mice (c–f) were administered papain intranasally every 3 days for the indicated time frame. a, b Flow cytometry analysis of TIGIT and IL-10-Venus expression by lineage-negative or positive cells (a) and ILC2s (CD45⁺ CD3^– CD19^– NK1.1^– CD11b^– Gr1⁻ Ter119^– CD127⁺ CD25⁺ ST2⁺) in the bronchoalveolar lavage (BAL) fluid (b) on the indicated day. c Flow cytometry analysis of expression of the indicated molecules by ILC2s in the BAL fluid on day 7. d Flow cytometry analysis of Ki67 expression by TIGIT⁺ (top) or TIGIT^– ILC2s (bottom) in the BAL fluid on day 7. e Flow cytometry analysis of TIGIT expression and the Live/Dead marker by ILC2s in the BAL fluid on days 7 and 16. f Quantitative RT-PCR analysis of the indicated gene transcripts in TIGIT^– and TIGIT⁺ ILC2s in the BAL fluid on day 7. For the flow cytometry plots, the numbers indicate the percentages of cells in each quadrant (a–c, e). In f, *p < 0.05 and **p < 0.01 by Student’s t-test. Data are representative of at least two independent experiments (mean ± s.d. of technical triplicates collected from 18 mice in f)