Fig. 3
From: Promoter-proximal pausing mediated by the exon junction complex regulates splicing
Mago controls chromatin accessibility. a Heatmaps of nucleosomal occupancy from MNase-seq experiments performed in biological duplicates from S2R+ cells in control or Mago knockdown conditions centered at the TSS in a ±1 Kb window. Rows indicate all the genes bound by Pol II and are sorted by decreasing Pol II occupancy. The color labels to the right indicate the levels of nucleosomal occupancy. Composite metagene profiles are also shown, with nucleosomal occupancy level on Y-axis and genomic coordinates on X-axis. b Genome browser view of averaged enrichment MNase-seq data from two independent biological duplicates in S2R+ cells treated with control or Mago double stranded RNA. The example shown here is Dbp80 gene, a well-defined pre-EJC target. c Log2 fold changes in nucleosomal occupancy at the TSS (250 bp upstream and downstream of TSS) after control and Mago knockdowns. The changes were separated according to pre-EJC binding and a two-sample t-test was performed. d Scatterplot between changes in nucleosome and Ser2P occupancy after either control or Mago knockdown. Pre-EJC-bound promoters are highlighted by orange color. A mild negative correlation, as shown in the indicated pearson coefficient of correlation, between nucleosome and Ser2P occupancies was found. e Log2 fold changes in K4Me3 levels normalized to the nucleosomal occupancy (MNase data) at the TSS (250 bp upstream and downstream of TSS), after control and Mago knockdowns. The changes were separated according to pre-EJC binding and a two-sample t-test was performed
