Fig. 4
From: Promoter-proximal pausing mediated by the exon junction complex regulates splicing
Mago controls chromatin accessibility and Pol II pausing in a gene size dependent manner. a, b Changes in nucleosome occupancy (a) and Ser2P levels (b) in control and Mago knockdown in S2R+ cells at the promoter, gene body, and TES, separated according to the size of the largest intron (ANOVA, p < 2.2 × 10−16). c Box plots showing changes in PRRs upon Mago depletion when compared to control, separated into different intron classes. d Percentage of pre-EJC-bound genes in different intron classes, along with the percentage of each class amongst all the expressed genes. The proportion of pre-EJC-bound genes is increased for genes containing larger introns, relative to their abundance. p-values are derived from Fisher’s exact test. e–g Venn diagrams showing the overlap between Mago-bound, Y14-bound, and eIF4A3-bound genes and genes with differential expression upon respective knockdowns. The overlap between genes that are downregulated upon each pre-EJC component knockdown, and their respective target genes is significant. p-values are derived from Fisher’s exact test. h Venn diagram showing the overlap between genes identified as differentially expressed in mRNA sequencing and nascent RNA sequencing (4sU-Seq) upon Mago KD. p-values are derived from Fisher’s exact test, separated for either upregulated or downregulated genes
