Fig. 8
From: Functional role of PGAM5 multimeric assemblies and their polymerization into filaments
PGAM5 localizes at inner mitochondrial membrane and at the membrane contact sites. a–d STED images of COS7 cells that were either a untransfected or b, c transiently transfected with wild type (WT) or F244E full-length myc-tagged PGAM5. Tom20 was visualized with anti-Tom20 antibody (green), Tim23 with anti-Tim23 antibody (green), and PGAM5 with anti-myc antibody (red). White arrows indicate contact sites between mitochondrial membranes in which myc-tagged PGAM5 was found colocalized with Tom20. Scale bars correspond to 5 µm in a, 5 µm in b, and 1 µm in c. d Upper panels show representative close-up views of PGAM5 localization to the sites of mitochondrial membrane contacts. White arrows indicate sites of PGAM5 (myc, red) overlap with the outer mitochondria membrane marker Tom20 (green)
