Fig. 1
From: Engineer chimeric Cas9 to expand PAM recognition based on evolutionary information
Chimeric Cas9 with expanded PAM specificities. a Design strategy for chimeric Cas9 (cCas9). b Sequence alignment of the 982–994 peptide fragment of orthologs. c Schematic of the EYFP reconstitution assay that was used to evaluate the cleavage activity of cCas9 variants at target sites containing indicated PAM sequences. d Data are shown as relative fluorescence intensity of EYFP measured by using flow cytometer 3 days after transfection into HEK293FT cells (n = 1). Source data are provided as a Source Data file
