Fig. 4
From: Engineer chimeric Cas9 to expand PAM recognition based on evolutionary information
Comparison of chimeric cCas9 variants at non-NNNRRN PAMs. a Direct comparison of v21-R, v21-L, N986S, v21-R-wt, v21-L-wt, N986S-wt, SaCas9, and SaCas9-KKH with eight plasmids doses across CCVACT, CCVGTG, CCVGTT, CCVGCT, CCVATT, CCVATG PAMs (V = A, C or G) by EYFP reconstitution assay. (n = 3) Error bars, s.e.m. The relative EYFP fluorescence intensity (y-axis) is measured by FACS. b Indel frequency induced by indicated variant at the sites where the third position of PAM is A or C. c Indel frequency induced by indicated variant at the sites where the third position of PAM is G. Each point represents the mean of one endogenous site (n = 3 independent biological replicates). The black line indicates the mean activities at all targets. a, b and c *P < 0.05 (paired t-test, two-tailed); **P < 0.01 (paired t-test, two-tailed); ***P < 0.001 (paired t-test, two-tailed); ****P < 0.0001 (paired t-test, two-tailed). Source data are provided as a Source Data file
