Fig. 3
From: A Rho-actin signaling pathway shapes cell wall boundaries in Arabidopsis xylem vessels
BDR recruits WAL to the boundaries of pit membranes. a BDR1 (pBDR1:BDR1-tagRFP) and MIDD1ΔN (pMIDD1:MIDD1ΔN-GFP) in metaxylem vessel cells in roots. The mid-plane is shown. BDR1-tagRFP (red arrowheads) localized to the boundary of the MIDD1ΔN-GFP domain (green arrowheads). The graph shows the intensity profile between the yellow arrowheads. The boxed area is magnified in the right panel. Yellow broken lines indicate edges of secondary cell walls (SW). The red broken line indicates the pit membrane. b WAL (pWAL:GFP-WAL) and BDR1 (pBDR1:BDR1-tagRFP) in metaxylem vessel cells in roots. Cortex (left) and mid-plane (right) from different cells are shown. c DIC of WT, bdr1-1, BDR3RNAi, and bdr1-1 BDR3RNAi plants. d Surface area and aspect ratio of secondary cell wall pits. Values are mean ± s.d. (n > 250 pits), *p < 0.05; **p < 0.01; ***p < 0.001 (ANOVA with Scheffe test). e Diagram of full-length and truncated fragments of WAL and BDR1. The black region indicates a DUF620 domain. Numbers indicate the positions of amino acid residues. f BiFC assay between WAL and BDR1 in leaf epidermal cells. BiFC signal was observed when nEYFP-WAL FL or nEYFP-WALΔN was co-expressed with BDR1 FL-cEYFP. g FRET efficiency between EYFP-WAL and BDR1-ECFP and between EYFP-WAL and truncated BDR1ΔC-ECFP. Values are mean ± s.d. (n > 15), ***p < 0.001 (Student’s t-test). h GFP-WAL in metaxylem vessel cells in roots of bdr1-1 BDR3RNAi plants treated with (+Est) or without (−Est) 2 μM estradiol. i Intensity ratio of GFP-WAL (inside of pit/outside of pit). Values are mean ± s.d. (n > 30), ***p < 0.001 (Student’s t-test). Bars = 50 µm (f) and 5 µm (a–c and h). Source data are provided as a Source Data file
