Fig. 2
From: Mitotic chromosome binding predicts transcription factor properties in interphase
Mitotic chromosome binding is correlated with TF-DNA co-localization in interphase. a Immunofluorescence labeling of H3K9me3 (Abcam, #ab8898) and Hoechst staining of a NIH-3T3 nucleus. Scale bar: 3 µm. b Automatic detection of regions displaying different densities of DNA: Heterochromatic (circled in red), DNA-rich (between red and yellow circles), or DNA-poor (circled in yellow). Scale bar: 3 µm. c Examples of TF-YPet interphase localization in NIH-3T3 cells, as compared to Hoechst staining and ranked by mitotic bound fraction (for n values see Supplementary Table 2). Bra Brachyury. Scale bar: 5 µm. d Correlation between the TF-YPet/Hoechst co-localization (MAFK: n = 8, NANOG and BHLHB8: n = 11, others: n = 10) and the mitotic bound fraction. e-g Correlation between the mitotic bound fraction and the fraction of YPet signal co-localized with heterochromatic regions (e), DNA-rich regions (f), and DNA-poor regions (g). n = 10. Error bars: SEM. r-values (r) and p-values (p) are based on Pearson correlation
