Fig. 5
Effect on the sensitivity to TB47 during the over-expression of CydABs in M. smegmatis. a The inhibition rates against M. smegmatis mc2 155 and derivative strains. Bacteria treated with different concentrations of TB47 and fluorescence units (FU) were recorded 4 h post-alamar blue addition. The inhibition rate was calculated as FUtreatment /FUuntreated × 100% and recorded for each strain per treatment. Data are expressed as mean ± SD from three independent repeats. b Inhibition growth of different M. smegmatis strains by TB47 at different concentrations. ①The plate lay-out is shown (M. smegmatis containing the construct detailed). ② to ④ showing the plates containing TB47 at 0, 0.1, and 1 (μg mL−1) incubated at 37 °C for 36 h. Msm, M. smegmatis; Mu, M. ulcerans; Mtb, M. tuberculosis; Mmr, M. marinum
