Table 1 Susceptibilities of different M. marinum/M. ulcerans strains to TB47 and rifampin in liquid using bioluminescence
Mycobacteria | Gene: qcrB from M. marinum | MICluxs (μg mL−1) | |
|---|---|---|---|
M. marinum strains | Â | TB47 | RIF |
Wild-type (Autoluminescent strain) | Wild-type | 0.001–0.008 | 0.25–1.0 |
Type 1 mutant | ACC to CCC (Thr323Pro) |  > 128 | 0.25–1.0 |
Type 2 mutant | ACC to GCC (Thr323Ala) |  > 128 | 0.25–1.0 |
Wild-type:: vector | Wild-type containing an empty plasmid | 0.001–0.008 | 0.25–1.0 |
Wild-type:: qcrB WT | Over-expression of wild-type qcrB | 32–64 | 0.25–1.0 |
Wild-type:: qcrB THR323PRO | Over-expression of qcrBThr323Pro |  > 128 | 0.25–1.0 |
Wild-type:: qcrB THR323ALA | Over-expression of qcrBThr323Ala |  > 128 | 0.5–2.0 |
M. ulcerans strains | Â | TB47 | RIF |
Wild-type (AlMu 1059) | Wild-type | 0.0016 | 0.06–0.25 |
Wild-type:: vector | Wild-type containing an empty plasmid | 0.0016–0.0031 | 0.06–0.25 |
Wild-type:: qcrB WT | Over-expression of wild-type qcrB | 0.05–0.10 | 0.06–0.25 |
Wild-type:: qcrB THR323PRO | Over-expression of qcrBThr323Pro | 0.10 to 0.40 | 0.06–0.25 |
Wild-type:: qcrB THR323ALA | Over-expression of qcrBThr323Ala | 0.10 to 0.40 | 0.125–0.5 |
