Fig. 5

MT dynamics and numbers are affected by the ER. a Kymographs recording the dynamic events of MT (GFP::TBA-1) and ER (mCherry::SP12) in 3° branches from a L4 stage WT animal. A blue box labels a +ER MT, while an orange box labels a –ER MT. MT’s plus end is marked by (+) and minus end is marked by (−). Scale bar, 5 μm and 15 s. b–e Quantification of dynamic parameters of MT plus ends (b, d) and minus ends (c, e). The frequency of rescue (b, c) and catastrophe (d, e) were plotted. Values are mean and error bars are SEM. At least 150 events from more than 30 worms are quantified. ns, not significant; *p < 0.05; **p < 0.01; ***p < 0.001 (Newman-Keuls multiple comparison test). f Confocal images showing tdTomato::PTRN-1 puncta in PVD 3° branches at adult stage. +ER (in blue box) means a 3° dendrite with ER present. -ER (in orange box) means the 3° branch without ER present. Scale bars, 10 μm. g Quantification of PTRN-1 puncta density in −ER and +ER 3° dendrites. Values are mean and error bars are SEM. At least 100 confocal views of PVD proximal regions from more than 40 worms are quantified. ***p < 0.001 (two-sided student’s t test)