Fig. 3
From: PTEN self-regulates through USP11 via the PI3K-FOXO pathway to stabilize tumor suppression
Usp11 ablation promotes tumor growth and metastasis in TRAMP mice. a Lysates from anterior prostate (AP) of 11-week-old Usp11+/Y and Usp11-/Y mice were subjected to immunoblotting. b Prostate tissue weight relative to body weight (BW) of 11-week-old Usp11+/Y (n = 8) and Usp11-/Y (n = 8) mice. VP, ventral prostate; AP, anterior prostate; DLP, dorsal-lateral prostate. c Ki-67-stained sections of anterior prostate lobes isolated from 11-week-old Usp11+/Y and Usp11-/Y mice (left). Quantification of the number of Ki-67-positive cells is shown in right. Green scale bars, 75μm; yellow scale bars, 150μm. d H&E-stained sections of dorsal-lateral prostate lobes isolated from 10-week-old TRAMP;Usp11+/Y (n = 8) and TRAMP;Usp11-/Y (n = 6) (left). Quantification of prostatic intraepithelial neoplasia (PIN) is shown in right. Scale bars, 75μm. e Ki-67-stained sections of anterior and ventral prostate lobes isolated from 10-week-old TRAMP;Usp11+/Y (n = 8) and TRAMP;Usp11-/Y (n = 6) (left). Quantification of the number of Ki-67-positive cells is shown in the right. Scale bars, 75μm. f MRI analysis of 20-week-old TRAMP;Usp11+/Y (n = 6) and TRAMP;Usp11-/Y (n = 6) mice (top), and quantification of prostate tumor volume using an Oxirix Imaging software (bottom, left). Prostate tissue weight of 25-week-old TRAMP;Usp11+/Y and TRAMP;Usp11-/Y mice is also shown (bottom, right). g Sections of prostate isolated from 25-week-old TRAMP;Usp11+/Y and TRAMP;Usp11-/Y mice stained with H&E and anti-SV40 T Ag. Scale bars, 75μm. h Sections of prostate isolated from 25-week-old TRAMP;Usp11+/Y and TRAMP;Usp11-/Y mice stained with H&E and anti-smooth muscle actin (SMA) (left). Percentage of invasive prostate carcinoma (CaP) in TRAMP;Usp11+/Y (n = 6) and TRAMP;Usp11-/Y (n = 6) mice is shown in the right. Scale bars, 75μm. i Sections of lymph nodes isolated from 25-week-old TRAMP;Usp11+/Y and TRAMP;Usp11-/Y mice stained with H&E and anti-SV40 T Ag (left). Percentage of lymph node (LN) metastases in TRAMP;Usp11+/Y (n = 6) and TRAMP;Usp11-/Y (n = 5) mice is shown in the right. Scale bars, 75μm. Error bars represent ± SEM. p Value was determined by Student’s t test (*p < 0.05; **p < 0.01; ***p < 0.001). H&E, hematoxylin and eosin; MRI, magnetic resonance imaging; TRAMP, transgenic adenocarcinoma mouse prostate
