Fig. 4
From: Combined loss of LAP1B and LAP1C results in an early onset multisystemic nuclear envelopathy
Cytoplasmic channels cross patient cell nuclei from top to bottom. Nuclear pore complexes in the nuclear envelope (NE) of control and patient-derived nuclei were stained by indirect immunofluorescence using mAb414 and imaged by confocal microscopy. Single optical sections are shown for one control and one patient-derived nucleus. A full z-stack of 27 optical sections, taken with a 70 nm step size through the patient-derived fibroblast, is shown on the right. Note the enlarged side views of the x and y axes demonstrating that the channel cuts across the whole nucleus. See Supplementary Fig. 5 for an assessment of nuclear pore complex distribution in the NE. Scale bar, 10 µm
