Fig. 3

Stability and controlled disassembly of RNA-DNA hybrid nanoshapes. a Temperature-dependent stability over time of purified RNA-DNA-11 hybrid nanoshapes (see Fig. 2a) analyzed by native PAGE in the presence of 2 mM magnesium salt. Discrete triangles and squares are stable at 20 °C but slowly re-equilibrate to the mixture of nanoshapes at 37 °C. C is a control containing the mixture of nanoshapes before separation of discrete structures (see Fig. 1d). b Polygonal nanoshapes, such as the triangle shown as an example, are disrupted by incubation at 37 °C with antisense RNA (red) that is fully complementary to the inner RNA strand. Mixtures of nanoshapes incubated with increasing amounts of antisense RNA were analyzed by PAGE (nanoshape:antisense ratios of 1:0.2, 1:0.5, 1:0.75, 1:1). Lower molecular weight fragments appear concurrently with the disassembly of the nanoshapes. Source data are provided as a Source Data file