Fig. 4
From: Versatile kit of robust nanoshapes self-assembling from RNA and DNA modules
RNA-DNA hybrid nanoshapes with extended DNA inserts. a The original RNA-DNA-11 nanoshape design obtained from screening (see Fig. 1d) was modified by extending the 11 bp double-stranded region of the DNA inserts by multiples of 10 base pairs corresponding to stepwise addition of 1–4 helix turns. Clean nanoshapes were formed with inserts up to 41 bp while an insert with 51 bp showed instability and aggregation in native PAGE analysis. C is a control containing the originally obtained mixture of nanoshapes that contain a DNA module with 11 bp in the double-stranded region (see Fig. 1d) A 50 bp DNA insert without RNA corner modules is shown for size comparison. b AFM topography images of hybrid nanoshapes with DNA inserts from samples used in PAGE analysis in panel a show the size increase of polygons in accord with extension of the base paired region of the DNA modules. Scale bars in upper left corner represent 10 nm. Scale bars with circular ends next to nanotriangles and nanosquares are shown corresponding to the approximate side length expected for nanoshapes containing the extended DNA inserts in all A-form conformation. Source data are provided as a Source Data file
