Fig. 2
PLCβ2 suppresses RNA virus-induced inflammation. a–c Q-PCR analysis of relative Tnf (a), Il6 (b), and Il12p40 (c) mRNA levels in skeletal muscle tissue from 14-day-old wild-type or Plcb2−/− mice infected with 50 μl of CVA16 virus (1.5 × 104 PFU/mouse) for 0 or 5 days. d, e Q-PCR analysis of relative Tnf (d) and Il6 (e) mRNA in wild-type or Plcb2−/− macrophages treated with poly(I:C) for the indicated times. f IB of lysates from wild-type or Plcb2−/− macrophages treated with poly(I:C) for the indicated times using anti-phospho-antibodies. g Q-PCR analysis of relative Plcb1-4 mRNA levels in mice peritoneal macrophages. h Q-PCR analysis of relative Tnf (h) and Il6 (i) mRNA in Plcb1-4 silence macrophages stimulated with poly(I:C) for the indicated times. *p < 0.05; **p < 0.01 and ****p < 0.0001 by unpaired t-test (a–e, h and i). Data are representative of three experiments with at least three independent biological replicates (mean and s.e.m. of n = 3 cultures in d–i or n = 3 mice per group in a–c)
