Fig. 3
From: 24-nt reproductive phasiRNAs are broadly present in angiosperms
Wide conservation of the miR2275/24-nt phasiRNA pathway in eudicots. a miR2275 diversity and 24-nt phasiRNA accumulation in grape. Above: the degree of sequence conservation along the miRNAs is represented by the density of blue color. Below: summed abundances of sRNAs at each of 42 24-PHAS loci in reads per ten million (RPTM). In the boxplot, the center line represents the median; box limits are the upper and lower quartiles; whiskers are the 1.5× interquartile range; points show the scatter of data points. b miR2275 diversity and 24-nt phasiRNA accumulation in strawberry, as in panel a, but for 221 24-PHAS loci. The boxplot is drawn as in panel a. c A polycistronic precursor encoding six MIR2275 stem–loops in strawberry, with miR2275 and miR2275* marked in red and orange, respectively. Bulges within the miR2275/miR2275* duplexes are indicated by a cyan circle. d A tree illustration of the conservation of the miR2275/24-nt phasiRNA pathway in plants. The plant phylogeny (left) was constructed according to the NCBI Taxonomy Common Tree. Plant orders or families denote representative plant species in a given order or family with genome sequenced and encoding miR2275 in the genome (black), with genome sequence but no miR2275 found (blue), and no genome sequenced (light gray). Numbers in parentheses after the family name indicate the number of plant species of that family used in this study. The presence of a given element (miR2275, DCL5, DCL3) or feature (MIR2275 in a polycistronic cluster) is indicated by colored boxes
