Fig. 1

Defective Th17 polarization of Hectd3-deficient CD4⁺ T cells ex vivo. a Representative flow cytometry analysis of intracellular IL-17A from in vitro polarized Hectd3^−/− and wild-type (WT) CD4⁺ T cells under Th0 or Th17 conditions, n = 3 per group, from three independent experiments. b Representative flow cytometry analysis of RORγt in in vitro polarized Hectd3^−/− and WT CD4⁺ T cells under Th0 or Th17 conditions, n = 3 per group, from three independent experiments. Th0 or Th17 conditions are described in Material and methods. c Quantification of a and b. Data are presented as mean ± SEM; p values were determined using Student’s t test. Source data are provided as a Source Data file. Gating strategy is shown in Supplementary Fig. 9