Fig. 2

Hectd3-deficient mice have attenuated EAE severity with reduced CD4⁺ T cell infiltration in the CNS. a EAE disease scores (mean ± SEM) of Hectd3^−/− (open) and wild-type (WT) (filled) mice following EAE induction, n = 6 per group, from three independent experiments. EAE induction was conducted as described in Material and methods. Description of clinical scores for EAE is presented in Material and methods. b Representative spinal cord and brain hematoxylin and eosin staining of Hectd3^−/− and WT mice 16 days following EAE induction; black bars represents 200 μm for spinal cord sections and 300 μm for brain sections; n = 4 per group, from three independent experiments. c Flow cytometry analysis of CD4⁺ and CD8⁺ T cells in the CNS of Hectd3^−/− and WT mice 13 days following EAE induction, n = 8 per group from three independent experiments. d Frequencies and absolute numbers (mean ± SEM) of the CNS CD4⁺ T cells in Hectd3^-−/− and WT mice, 13 days following EAE induction. e Flow cytometry analysis of CD4⁺ and CD8⁺ T cells in draining lymph nodes (dLNs) of Hectd3^−/− and WT mice 13 days following EAE induction; n = 10 per group from three independent experiments. f Frequencies and absolute numbers (mean ± SEM) of CD4⁺ T cells in the dLNs of Hectd3^−/− and WT mice, 13 days following EAE induction, n = 7 per group from three independent experiments. c–f, Data (n = 6–10) are representative of three independent experiments and are presented as mean ± SEM; p value was obtained using Mann–Whitney two-tailed test for the EAE clinical scores and Student’s two-tailed t test for all other data. Source data are provided as a Source Data file. Gating strategy is shown in Supplementary Fig. 9