Fig. 9 | Nature Communications

Fig. 9

From: Hectd3 promotes pathogenic Th17 lineage through Stat3 activation and Malt1 signaling in neuroinflammation

Fig. 9

Stat3 linker region and Hectd3 DOC domain mediate the interaction between Stat3 and Hectd3. a Immunoblot of Xpress and Flag following Flag or Xpress immunoprecipitation of protein extracts from HEK293T cells co-transfected as indicated with HA-Ub, Flag-Stat3, and Xpress-Hectd3; data are representative of three independent experiments. b Stat3 and Hectd3 domains representation. c Immunoblot of Xpress, Flag, and GAPDH in the Flag-immunoprecipitate or input of protein extracts from HEK293T cells co-transfected as indicated with Flag-Stat3, Flag-Stat3 ΔND, Flag-Stat3 ΔCCD, Flag-Stat3 ΔDBD, Flag-Stat3 ΔLD, Flag-Stat3 ΔSH2D, or Flag-Stat3 ΔTAD, and Xpress-Hectd3; data are representative of two independent experiments. * denotes non-specific bands. d Immunoblot of Flag, Xpress, and GAPDH in the Xpress-immunoprecipitate or input of protein extracts from HEK293T cells co-transfected as indicated, with Xpress-Hectd3, Xpress-Hectd3 ΔND, Xpress-Hectd3 ΔDOC, Xpress-Hectd3 ΔLD, or Xpress-Hectd3 ΔHECT, and Flag-Stat3; data are representative of two independent experiments. Source data are provided as a Source Data file

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