Fig. 6

WT does not complement PDEB1 KO in vitro or in vivo. a Proportion of dsRed-positive cells in suspension culture. Fluorescence was monitored by flow cytometry (Supplementary Figure 2). Tagged:untagged cells were mixed at a ratio of 2:1. b WT-dsRed or KO-dsRed was mixed with untagged WT at a ratio of 2:1 and tested for SoMo. Top panel: WT + WT-dsRed co-culture. WT-dsRed parasites are evenly distributed throughout the projections. Lower panel: KO + WT-dsRed co-culture. KO-dsRed cells form a gradient with a high proportion of KO-dsRed cells at the base of the projection and progressively fewer cells extending to the tip. c Prevalence and intensities of midgut infections at day 14 post infection. P-value is shown for Fisher’s exact test, two-sided. d Proventriculus infection rates at day 14 post infection. In the mixed infections, only dsRed cells were scored. P-value is shown for Fisher’s exact test, two-sided