Fig. 6

Itk^−/− mice have impaired intestinal tissue integrity. a, b In total, 3.0% DSS-dissolved water was given to WT and Itk^−/− mice for 5 days and then changed to normal drinking water for 10 days (a). Weight changes of treated mice were monitored daily (b). c, d The proportion of amphiregulin-producing Sca-1⁺ ILC (lineage-negative NK1.1⁻ NKP46⁻) from siLP and cLP tissue from WT and Itk^−/− mice in steady-state conditions. e The survival of 3.0% DSS-treated WT and Itk^−/− mice from D0 to D15 is shown. f–j WT and Itk^−/− mice were treated with 3.0% DSS water for 7 days and histology of distal colon was examined (f). The length of colons from each group was measured (g) and colon ILC2 proportions were examined at D7 (h). Isolated lymphocytes from colon LP of WT and Itk^−/− mice at D7 were stimulated with PMA and ionomycin for 5 h and stained for IL-5 and IL-13. Data show cells gated on lineage-negative CD90⁺ Sca-1⁺ cells (i). Complied data are shown (j). k Intestinal permeability of WT and Itk^−/− mice at steady-state was measured by assessing serum levels of FITC-dextran 4 h after oral administration. Data are the compilation of two independent experiments using WT (n = 4-6) and Itk^−/− mice (n = 4-8). Bar graphs with error bars show the averages and the SEMs. Statistical significance was analyzed using Student’s t tests (**p < 0.01; ***p < 0.001). Source data are provided as a Source Data file