Fig. 5
Halorubrum virus membrane fusion. a HPRV-6 fusion kinetics with cells. Fluorescently labelled HRPV-6 was incubated at 37 °C with Halorubrum sp. SS7-4 host cells (grey dots) or the non-host cells Halorubrum PV6 (white dots) at 37 °C under continuous stirring and dequenching measured at 585 nm. As additional controls, HPRV-6 was incubated with Halorubrum sp. SS7-4 host cells at 4 °C (white boxes) or spikeless HRPV-6 was prepared by proteinase K digestion (TCPK) and incubated with the same host cells at 37 °C (black triangles). b HPRV-6 fusion kinetics with liposomes derived from haloarchael SS7-4 cell lipids. Fluorescently labelled HRPV-6 was incubated with liposomes at 37 °C (white boxes) and 55 °C (grey dots). As a control, spikeless HRPV-6 was incubated with haloarchaeal liposomes at 55 °C (white triangles). Results from A and B are representative for n = 3 independent experiments. c Lipid mixing kinetics of fluorescently labelled HRPV-6 particles after incubation with liposomes at 45 °C (black dots), 50 °C (white triangles) and 55 °C (grey dots) normalized to the maximum extend of fusion at 55 °C. Each segmented line represent the single exponential fit to the kinetic data. Source data are provided as a Source Data file
