Fig. 7
Comparative enzymatic cascade reaction for glucose detection. a Scheme of the cascade reaction with continuous O2-purging in a batch mode. b The concentration-response curve with the linear calibration plots (inset) and colour change (inset) for glucose detection in batch reactor. The AKCN was employed as an oxidase (0.1 M phosphate buffer, pH 7) and a peroxidase (0.1 M acetate buffer pH 4), sequentially in cascade reaction. c Scheme of the cascade reaction in a microfluidic device and actual device image (inset). d TMB oxidation by AKCN in the microfluidic channel (monitored as a function of time) for glucose detection in the concentration range of 0.01 mM–1.0 M. e The linear calibration for initial TMB oxidation rate (vi) vs. glucose concentration. The error bar represents the standard deviation from the repeated experiment after three times. Source data are provided as a Source Data file
