Fig. 5
From: Morphologic determinant of tight junctions revealed by claudin-3 structures
Morphology and adhesivity of TJ strands formed by mCldn3 with a different TM3 structure. a Adhesive property of TJs formed by a stable SF7-derived cell line expressing mCldn3. Dissociation assay showed that TJs formed by mCldn3 P134G or P134A had stronger adhesivity than TJs of mCldn3 WT (n = 18/group). Results from three distinct clones are shown as means ± SEM. P-values were calculated using a two-tailed independent t-test, and P < 0.05 was considered significant. n.s., not significant; ***p < 0.001. b–d Representative electron microscopic images of freeze-fracture replicas of TJ strands of mCldn3. b TJ strands consisting of wild-type mCldn3 appear as straight strands (arrow). c TJ strands consisting of mCldn3 P134G appear as curvy strands with many hairpin curves (arrowhead). d TJ strands consisting of mCldn3 P134A appear as curvy strands with many hairpin curves. PF = protoplasmic face. EF = exoplasmic face. Scale bar = 500 nm. Source data are provided as a Source Data file (a)
