Fig. 2
From: Single gold-bridged nanoprobes for identification of single point DNA mutations
Fabrication of single nanoparticle (NP) biosensor of high sensitivity and fidelity. a Schematic illustration of single NP sensing (sNPS) for identifying single point DNA mutations. b Representative Rayleigh light scattering (RLS) spectra and in-situ dark-field microscopy image of an Au-bridged NP. Scale bar, 1 μm. c Localized surface plasmon resonance (LSPR) λmax shifts upon binding of DNA and MutS. Same line legend for (b, c). Each step of molecular binding (1, 2, and 3) illustrated in (d). Insets: real-time images of a single NP obtained with charge-coupled device (CCD) camera. e Concentration of MutS for adequate signal response. Calibration curve showing the linear relationship between the Δλmax and various concentrations of MutS. f Limit of detection (LOD) of the mutant DNA target. Calibration curve showing the linear relationship between the Δλmax and various concentrations of target DNA. For all panels, error bars represent mean ± S.E.M. All experiments were performed in triplicate. Source data of Fig. 2b, c, e, and f are provided as a Source Data file
