Fig. 4
TG-interacting factor 1 (Tgif1) is dispensable for the bone anabolic function of canonical Wnt signaling. a Representative images of von Kossa-stained histological sections and fluorescence double labeling to visualize bone formation (insets) of the proximal tibiae of 12-week-old Tgif1+/+ and Tgif1−/− male mice after treatment with anti-sclerostin antibody (Scl-Ab) or vehicle (Veh). Scale bars indicate 1 mm (black) and 50 μm (white). b Histomorphometric analysis of the proximal tibiae of 12-week-old male Tgif1+/+ and Tgif1−/− mice after treatment with Scl-Ab or Veh (Tgif1+/++Veh: N = 12, Tgif1+/++Scl-Ab: N = 9, Tgif1−/−+Veh: N = 6, Tgif1−/−+Scl-Ab: N = 8). For abbreviations see the legend to Fig. 1. **p< 0.01, ***p< 0.001 vs. Veh-treated group of the same genotype. Error bars represent the s.e.m. Statistical analysis was performed using analysis of variance (ANOVA) followed by Newman–Keuls post-hoc test
