Fig. 3 | Nature Communications

Fig. 3

From: Promiscuous enzymatic activity-aided multiple-pathway network design for metabolic flux rearrangement in hydroxytyrosol biosynthesis

Fig. 3

Directed divergent evolution of HpaBC. a Reactions targeted for directed divergent evolution; b Periodate assay of expected substrates and products of mutant HpaBCs. c Standard curves of the periodate assay; d Flowchart of the high-throughput screening of HpaBC; e OD400 of the periodate assay of HpaBC mutants selected after positive screening with tyrosol and negative screening with tyramine; f OD400 of the periodate assay of HpaBC mutants selected after positive screening with tyramine and negative screening with tyrosol. WT, wild-type. The data shown in e and f are from three replicate experiments and are expressed as the mean ± SD. Source data of b, c, e and f are provided in the Excel format Source Data file

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