Table 1 Early flux analysis of the combined pathway, using tyramine as a substrate

From: Promiscuous enzymatic activity-aided multiple-pathway network design for metabolic flux rearrangement in hydroxytyrosol biosynthesis

Time (h)

0

1

2

3

4

5

6

Tyramine (mM)

15.00

14.99 ± 0.0005

14.98 ± 0.0008

14.96 ± 0.0006

14.70 ± 0.05

14.40 ± 0.07

13.90 ± 0.008

Dopamine (mM)

0

0

0

0

0

0

0.05 ± 0.0008

3,4-DHPAA (mM)

0

0

0

0

0

0

0

4HPAA (mM)

0

0

0

0.10 ± 0.002

0.21 ± 0.009

0.12 ± 0.0004

0

Tyrosol (mM)

0

0.01 ± 0.0003

0.02 ± 0.0004

0.05 ± 0.0007

0.13 ± 0.002

0.52 ± 0.006

0

Hydroxytyrosol (mM)

0

0

0

0

0

0

1.12 ± 0.006

  1. ±Values represent standard deviations from three independent data points