Fig. 7
From: Allosteric enhancement of ORP1-mediated cholesterol transport by PI(4,5)P2/PI(3,4)P2
Key regions for membrane association of ORP1-ORD. a Liposome association assay for ORP1-ORD. b Potential membrane-binding sites on the open ORP1-ORD structure. The surface representation is colored according to electrostatic potential (positive, blue; negative, red), and the lid region and four basic patches are circled. c Bar graph for membrane-bound fractions of basic region mutants to liposome enriched in negatively charged lipids. d Effects of basic region mutations on the initial rates of PI(3,4)P2-dependent DHE transport. (For all bar graphs, data are shown as mean ± s.e.m. (error bar), n = 3)
